Protein functions and interactions


We have developed a new technique to predict protein (enzymatique) function, protein-protein and protein-chemical interactions using the signature descriptor described in the QSAR pages. The signature of a protein sequence (or DNA sequence) is a vector of occurence numbers of the sequence k-mers (or k-words). To predict protein binding pairs a new type of kernel was developed where the signagture is a product of the signatures of both the bait (target) and the prey (ligand). The kernel, which is used with SVM compares well in term of accuracy/precision/sensitivity with other existing techniques. Our method is not specific to a particular type of experimental dataset, does not require knowledge of protein domains or physico-chemical parameters, it just uses as input a list of sequences and binding pairs. More information can be found in the following papers:
 
  1. Faulon J.L., Misra M., Martin S., Sale, K., Sapra R.. Genome Scale Enzyme-metabolites and Drug-Target interaction predictions using the signature molecular descriptor, Bioinformatics in press 2007 [PMID: 18037612 ] (link to journal)
  2. Martin S., Brown W. M., Faulon J.L. Using Product Kernels to Predict Protein Interactions. In Advances in Biochemical Engineering/Biotechnology, Eds. H. Seitz and M. Werther, Springer-Verlag. in press, 2007 [PMID: 17922100]
  3. Brown W. M., Martin S., Chabarek J.P., Strauss C, Faulon J.L. Prediction of β-Strand Packing Interactions using the Signature Product, Journal of Molecular Modeling, in press. [PMID: 16365772]   (link to journal)
  4. Martin S., Roe D., Faulon J.L. Predicting Protein-Protein Interactions using Signature Products, Bioinformatics, 21, 218-26, 2005. [PMID: 15319262]. (.pdf manuscript).
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